Transmission microsphere‐assisted dark‐field microscopy

Webpage : https://onlinelibrary.wiley.com/doi/abs/10.1002/pssr.201800445

Microsphere-assisted microscopy allows the limit of the diffraction of light to be overcome while being noninvasive, full-field, label-free and easy-to-implement. However, the observation of translucent samples remains difficult using a classical bright-field illumination. In this work, a method is presented for the inspection of quasi-transparent sub-diffraction-limited structures by using dark-field illumination in the transmission mode. Glass-imprint features, having a size of 250 nm, as well as fixed mouse brain cells have been visualized using the dark-field microsphere-assisted technique. The possibility to observe feature sizes up to 100 nm has been demonstrated in air using a 25-µm-diameter glass microsphere combined with an optical microscope, opening new possibilities for biological imaging.

S. Perrin , H. Li , K. Badu , T. Comparon , N. Lemercier , P. Montgomery , J. Vonesch , S. Lecler

Physica Status Solidi - Rapid Research Letters , Volume 13 , Number 2 , page 1800445 - 2018

@Article{2-PLBC18,
 author = {Perrin, S. and Li, H. and Badu, K. and Comparon, T. and Lemercier, N. and Montgomery, P. and Vonesch, J. and Lecler, S.},
 title = {Transmission microsphere‐assisted dark‐field microscopy},
 journal = {Physica Status Solidi - Rapid Research Letters},
 number = {2},
 volume = {13},
 pages = {1800445},
 month = {Oct},
 year = {2018},
 keywords = {Super-r\'esolution; Microscopy; Dark-field; Microsphere},
 doi = {10.1002/pssr.201800445},
 x-international-audience = {Yes},
 x-language = {EN},
 url = {http://publis.icube.unistra.fr/2-PLBC18}
}